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Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Fluorescence microscopy is employed to identify Kinesin-1 cargos. Recently, the heavy chain of Kinesin-1 (KIF5B) was shown to transport the nuclear transcription factor c-MYC for proteosomal degradation in the cytoplasm. The method described here involves the study of a motorless KIF5B mutant for fl...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MyJove Corporation
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828159/ https://www.ncbi.nlm.nih.gov/pubmed/26966786 http://dx.doi.org/10.3791/53632 |