Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria

Cystic fibrosis (CF) is the most common hereditary lung disease in the Caucasian population, characterized by viscous bronchial secretion, consecutive defective mucociliary clearance, and unavoidable colonization with microorganisms. Besides Pseudomonasaeruginosa, Staphylococcusaureus is the most co...

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Autores principales: Masoud-Landgraf, Lilian, Zarfel, Gernot, Kaschnigg, Tanja, Friedl, Simone, Feierl, Gebhard, Wagner-Eibel, Ute, Eber, Ernst, Grisold, Andrea J., Kittinger, Clemens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828482/
https://www.ncbi.nlm.nih.gov/pubmed/26821237
http://dx.doi.org/10.1007/s00284-016-0994-z
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author Masoud-Landgraf, Lilian
Zarfel, Gernot
Kaschnigg, Tanja
Friedl, Simone
Feierl, Gebhard
Wagner-Eibel, Ute
Eber, Ernst
Grisold, Andrea J.
Kittinger, Clemens
author_facet Masoud-Landgraf, Lilian
Zarfel, Gernot
Kaschnigg, Tanja
Friedl, Simone
Feierl, Gebhard
Wagner-Eibel, Ute
Eber, Ernst
Grisold, Andrea J.
Kittinger, Clemens
author_sort Masoud-Landgraf, Lilian
collection PubMed
description Cystic fibrosis (CF) is the most common hereditary lung disease in the Caucasian population, characterized by viscous bronchial secretion, consecutive defective mucociliary clearance, and unavoidable colonization with microorganisms. Besides Pseudomonasaeruginosa, Staphylococcusaureus is the most common bacterial species colonizing the CF respiratory tract. Under antibiotic pressure S.aureus is able to switch to small colony variants (SCV). These small colony variants can invade epithelial cells, overcome antibiotic therapy inside the cells and can be the starting point for extracellular recolonization. The aim of the present study was the isolation and characterization of S. aureus small colony variants from Austrian cystic fibrosis patients. Samples collected from 147 patients were screened for the presence of S. aureus wild-type and small colony variants. Antibiotic susceptibility testing and determination of the small colony variants causing auxotrophism were performed. Wild-type isolates were assigned to corresponding small colony variants with spa typing. In total, 17 different small colony variant isolates and 12 corresponding wild-type isolates were obtained. 13 isolates were determined thymidine auxotroph, 2 isolates were auxotroph for hemin, and none of the tested isolates was auxotroph for both, respectively. The presence of SCVs is directly related to a poor clinical outcome, therefore a monitoring of SCV prevalence is recommended. This study revealed rather low SCV ratios in CF patients compared to other countries.
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spelling pubmed-48284822016-04-21 Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria Masoud-Landgraf, Lilian Zarfel, Gernot Kaschnigg, Tanja Friedl, Simone Feierl, Gebhard Wagner-Eibel, Ute Eber, Ernst Grisold, Andrea J. Kittinger, Clemens Curr Microbiol Article Cystic fibrosis (CF) is the most common hereditary lung disease in the Caucasian population, characterized by viscous bronchial secretion, consecutive defective mucociliary clearance, and unavoidable colonization with microorganisms. Besides Pseudomonasaeruginosa, Staphylococcusaureus is the most common bacterial species colonizing the CF respiratory tract. Under antibiotic pressure S.aureus is able to switch to small colony variants (SCV). These small colony variants can invade epithelial cells, overcome antibiotic therapy inside the cells and can be the starting point for extracellular recolonization. The aim of the present study was the isolation and characterization of S. aureus small colony variants from Austrian cystic fibrosis patients. Samples collected from 147 patients were screened for the presence of S. aureus wild-type and small colony variants. Antibiotic susceptibility testing and determination of the small colony variants causing auxotrophism were performed. Wild-type isolates were assigned to corresponding small colony variants with spa typing. In total, 17 different small colony variant isolates and 12 corresponding wild-type isolates were obtained. 13 isolates were determined thymidine auxotroph, 2 isolates were auxotroph for hemin, and none of the tested isolates was auxotroph for both, respectively. The presence of SCVs is directly related to a poor clinical outcome, therefore a monitoring of SCV prevalence is recommended. This study revealed rather low SCV ratios in CF patients compared to other countries. Springer US 2016-01-28 2016 /pmc/articles/PMC4828482/ /pubmed/26821237 http://dx.doi.org/10.1007/s00284-016-0994-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Masoud-Landgraf, Lilian
Zarfel, Gernot
Kaschnigg, Tanja
Friedl, Simone
Feierl, Gebhard
Wagner-Eibel, Ute
Eber, Ernst
Grisold, Andrea J.
Kittinger, Clemens
Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title_full Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title_fullStr Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title_full_unstemmed Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title_short Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
title_sort analysis and characterization of staphylococcus aureus small colony variants isolated from cystic fibrosis patients in austria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828482/
https://www.ncbi.nlm.nih.gov/pubmed/26821237
http://dx.doi.org/10.1007/s00284-016-0994-z
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