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A redesigned CRISPR/Cas9 system for marker-free genome editing in Plasmodium falciparum
BACKGROUND: A highly efficient CRISPR/Cas9-based marker-free genome editing system has been established in Plasmodium falciparum (Pf). However, with the current methods, two drug-selectable markers are needed for episome retention, which may present hurdles for consecutive genome manipulations due t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828878/ https://www.ncbi.nlm.nih.gov/pubmed/27066899 http://dx.doi.org/10.1186/s13071-016-1487-4 |