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A redesigned CRISPR/Cas9 system for marker-free genome editing in Plasmodium falciparum

BACKGROUND: A highly efficient CRISPR/Cas9-based marker-free genome editing system has been established in Plasmodium falciparum (Pf). However, with the current methods, two drug-selectable markers are needed for episome retention, which may present hurdles for consecutive genome manipulations due t...

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Detalles Bibliográficos
Autores principales: Lu, Junnan, Tong, Ying, Pan, Jiaqiang, Yang, Yijun, Liu, Quan, Tan, Xuefang, Zhao, Siting, Qin, Li, Chen, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828878/
https://www.ncbi.nlm.nih.gov/pubmed/27066899
http://dx.doi.org/10.1186/s13071-016-1487-4