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Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands

OBJECTIVES: Intratypic molecular variants of human papillomavirus (HPV) type-16 and -18 exist. In the Netherlands, a bivalent vaccine, composed of recombinant L1 proteins from HPV-16 and -18, is used to prevent cervical cancer since 2009. Long-term vaccination could lead to changes in HPV-16 and -18...

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Autores principales: King, Audrey J., Sonsma, Jan A., Vriend, Henrike J., van der Sande, Marianne A. B., Feltkamp, Mariet C., Boot, Hein J., Koopmans, Marion P. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829201/
https://www.ncbi.nlm.nih.gov/pubmed/27070907
http://dx.doi.org/10.1371/journal.pone.0152782
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author King, Audrey J.
Sonsma, Jan A.
Vriend, Henrike J.
van der Sande, Marianne A. B.
Feltkamp, Mariet C.
Boot, Hein J.
Koopmans, Marion P. G.
author_facet King, Audrey J.
Sonsma, Jan A.
Vriend, Henrike J.
van der Sande, Marianne A. B.
Feltkamp, Mariet C.
Boot, Hein J.
Koopmans, Marion P. G.
author_sort King, Audrey J.
collection PubMed
description OBJECTIVES: Intratypic molecular variants of human papillomavirus (HPV) type-16 and -18 exist. In the Netherlands, a bivalent vaccine, composed of recombinant L1 proteins from HPV-16 and -18, is used to prevent cervical cancer since 2009. Long-term vaccination could lead to changes in HPV-16 and -18 virus population, thereby hampering vaccination strategies. We determined the genetic diversity of the L1 gene in HPV-16 and -18 viral strains circulating in the Netherlands at the start of vaccination in order to understand the baseline genetic diversity in the Dutch population. METHODS: DNA sequences of the L1 gene were determined in HPV-16 (n = 241) and HPV-18 (n = 108) positive anogenital samples collected in 2009 and 2011 among Dutch 16- to 24-year old female and male attendees of the sexually transmitted infection (STI) clinics. Phylogenetic analysis was performed and sequences were compared to reference sequences HPV-16 (AF536179) and HPV-18 (X05015) using BioNumerics 7.1. RESULTS: For HPV-16, ninety-five single nucleotide polymorphism (SNPs) were identified, twenty–seven (28%) were non-synonymous variations. For HPV-18, seventy-one SNPs were identified, twenty-nine (41%) were non-synonymous. The majority of the non-silent variations were located in sequences encoding alpha helix, beta sheet or surface loops, in particular in the immunodominant FG loop, and may influence the protein secondary structure and immune recognition. CONCLUSIONS: This study provides unique pre-vaccination/baseline data on the genetic L1 diversity of HPV-16 and -18 viruses circulating in the Netherlands among adolescents and young adults.
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spelling pubmed-48292012016-04-22 Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands King, Audrey J. Sonsma, Jan A. Vriend, Henrike J. van der Sande, Marianne A. B. Feltkamp, Mariet C. Boot, Hein J. Koopmans, Marion P. G. PLoS One Research Article OBJECTIVES: Intratypic molecular variants of human papillomavirus (HPV) type-16 and -18 exist. In the Netherlands, a bivalent vaccine, composed of recombinant L1 proteins from HPV-16 and -18, is used to prevent cervical cancer since 2009. Long-term vaccination could lead to changes in HPV-16 and -18 virus population, thereby hampering vaccination strategies. We determined the genetic diversity of the L1 gene in HPV-16 and -18 viral strains circulating in the Netherlands at the start of vaccination in order to understand the baseline genetic diversity in the Dutch population. METHODS: DNA sequences of the L1 gene were determined in HPV-16 (n = 241) and HPV-18 (n = 108) positive anogenital samples collected in 2009 and 2011 among Dutch 16- to 24-year old female and male attendees of the sexually transmitted infection (STI) clinics. Phylogenetic analysis was performed and sequences were compared to reference sequences HPV-16 (AF536179) and HPV-18 (X05015) using BioNumerics 7.1. RESULTS: For HPV-16, ninety-five single nucleotide polymorphism (SNPs) were identified, twenty–seven (28%) were non-synonymous variations. For HPV-18, seventy-one SNPs were identified, twenty-nine (41%) were non-synonymous. The majority of the non-silent variations were located in sequences encoding alpha helix, beta sheet or surface loops, in particular in the immunodominant FG loop, and may influence the protein secondary structure and immune recognition. CONCLUSIONS: This study provides unique pre-vaccination/baseline data on the genetic L1 diversity of HPV-16 and -18 viruses circulating in the Netherlands among adolescents and young adults. Public Library of Science 2016-04-12 /pmc/articles/PMC4829201/ /pubmed/27070907 http://dx.doi.org/10.1371/journal.pone.0152782 Text en © 2016 King et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
King, Audrey J.
Sonsma, Jan A.
Vriend, Henrike J.
van der Sande, Marianne A. B.
Feltkamp, Mariet C.
Boot, Hein J.
Koopmans, Marion P. G.
Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title_full Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title_fullStr Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title_full_unstemmed Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title_short Genetic Diversity in the Major Capsid L1 Protein of HPV-16 and HPV-18 in the Netherlands
title_sort genetic diversity in the major capsid l1 protein of hpv-16 and hpv-18 in the netherlands
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829201/
https://www.ncbi.nlm.nih.gov/pubmed/27070907
http://dx.doi.org/10.1371/journal.pone.0152782
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