Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids. The efficiency of co-transformation cloning is however low and the Gibson assembly reagents are expensi...

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Detalles Bibliográficos
Autores principales: Benoit, Roger M., Ostermeier, Christian, Geiser, Martin, Li, Julia Su Zhou, Widmer, Hans, Auer, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830597/
https://www.ncbi.nlm.nih.gov/pubmed/27073895
http://dx.doi.org/10.1371/journal.pone.0153158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830597/
https://www.ncbi.nlm.nih.gov/pubmed/27073895
http://dx.doi.org/10.1371/journal.pone.0153158

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