Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum

BACKGROUND: Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a single round of mutagenesis including targeted disruption. Although the eff...

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Detalles Bibliográficos
Autores principales: Mukai, Asuka, Ichiraku, Aya, Horikawa, Kazuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831088/
https://www.ncbi.nlm.nih.gov/pubmed/27075750
http://dx.doi.org/10.1186/s12896-016-0267-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831088/
https://www.ncbi.nlm.nih.gov/pubmed/27075750
http://dx.doi.org/10.1186/s12896-016-0267-8

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