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Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis

The Rho-family GTPase Cdc42 regulates cell polarity and localizes to the cell division site. Cdc42 is activated by guanine nucleotide exchange factors (GEFs). We report that Cdc42 promotes cytokinesis via a unique spatiotemporal activation pattern due to the distinct action of its GEFs, Gef1 and Scd...

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Autores principales: Wei, Bin, Hercyk, Brian S., Mattson, Nicholas, Mohammadi, Ahmad, Rich, Julie, DeBruyne, Erica, Clark, Mikayla M., Das, Maitreyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831878/
https://www.ncbi.nlm.nih.gov/pubmed/26941334
http://dx.doi.org/10.1091/mbc.E15-10-0700
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author Wei, Bin
Hercyk, Brian S.
Mattson, Nicholas
Mohammadi, Ahmad
Rich, Julie
DeBruyne, Erica
Clark, Mikayla M.
Das, Maitreyi
author_facet Wei, Bin
Hercyk, Brian S.
Mattson, Nicholas
Mohammadi, Ahmad
Rich, Julie
DeBruyne, Erica
Clark, Mikayla M.
Das, Maitreyi
author_sort Wei, Bin
collection PubMed
description The Rho-family GTPase Cdc42 regulates cell polarity and localizes to the cell division site. Cdc42 is activated by guanine nucleotide exchange factors (GEFs). We report that Cdc42 promotes cytokinesis via a unique spatiotemporal activation pattern due to the distinct action of its GEFs, Gef1 and Scd1, in fission yeast. Before cytokinetic ring constriction, Cdc42 activation, is Gef1 dependent, and after ring constriction, it is Scd1 dependent. Gef1 localizes to the actomyosin ring immediately after ring assembly and promotes timely onset of ring constriction. Gef1 is required for proper actin organization during cytokinesis, distribution of type V myosin Myo52 to the division site, and timely recruitment of septum protein Bgs1. In contrast, Scd1 localizes to the broader region of ingressing membrane during cytokinetic furrowing. Scd1 promotes normal septum formation, and scd1Δ cells display aberrant septa with reduced Bgs1 localization. Thus we define unique roles of the GEFs Gef1 and Scd1 in the regulation of distinct events during cytokinesis. Gef1 localizes first to the cytokinetic ring and promotes timely constriction, whereas Scd1 localizes later to the ingressing membrane and promotes septum formation. Our findings are consistent with reports that complexity in GTPase signaling patterns enables exquisite precision over the control of cellular processes.
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spelling pubmed-48318782016-06-30 Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis Wei, Bin Hercyk, Brian S. Mattson, Nicholas Mohammadi, Ahmad Rich, Julie DeBruyne, Erica Clark, Mikayla M. Das, Maitreyi Mol Biol Cell Articles The Rho-family GTPase Cdc42 regulates cell polarity and localizes to the cell division site. Cdc42 is activated by guanine nucleotide exchange factors (GEFs). We report that Cdc42 promotes cytokinesis via a unique spatiotemporal activation pattern due to the distinct action of its GEFs, Gef1 and Scd1, in fission yeast. Before cytokinetic ring constriction, Cdc42 activation, is Gef1 dependent, and after ring constriction, it is Scd1 dependent. Gef1 localizes to the actomyosin ring immediately after ring assembly and promotes timely onset of ring constriction. Gef1 is required for proper actin organization during cytokinesis, distribution of type V myosin Myo52 to the division site, and timely recruitment of septum protein Bgs1. In contrast, Scd1 localizes to the broader region of ingressing membrane during cytokinetic furrowing. Scd1 promotes normal septum formation, and scd1Δ cells display aberrant septa with reduced Bgs1 localization. Thus we define unique roles of the GEFs Gef1 and Scd1 in the regulation of distinct events during cytokinesis. Gef1 localizes first to the cytokinetic ring and promotes timely constriction, whereas Scd1 localizes later to the ingressing membrane and promotes septum formation. Our findings are consistent with reports that complexity in GTPase signaling patterns enables exquisite precision over the control of cellular processes. The American Society for Cell Biology 2016-04-15 /pmc/articles/PMC4831878/ /pubmed/26941334 http://dx.doi.org/10.1091/mbc.E15-10-0700 Text en © 2016 Wei et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Wei, Bin
Hercyk, Brian S.
Mattson, Nicholas
Mohammadi, Ahmad
Rich, Julie
DeBruyne, Erica
Clark, Mikayla M.
Das, Maitreyi
Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title_full Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title_fullStr Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title_full_unstemmed Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title_short Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis
title_sort unique spatiotemporal activation pattern of cdc42 by gef1 and scd1 promotes different events during cytokinesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831878/
https://www.ncbi.nlm.nih.gov/pubmed/26941334
http://dx.doi.org/10.1091/mbc.E15-10-0700
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