Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast

Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However,...

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Autores principales: Miao, Yansong, Han, Xuemei, Zheng, Liangzhen, Xie, Ying, Mu, Yuguang, Yates, John R., Drubin, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832064/
https://www.ncbi.nlm.nih.gov/pubmed/27068241
http://dx.doi.org/10.1038/ncomms11265
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author Miao, Yansong
Han, Xuemei
Zheng, Liangzhen
Xie, Ying
Mu, Yuguang
Yates, John R.
Drubin, David G.
author_facet Miao, Yansong
Han, Xuemei
Zheng, Liangzhen
Xie, Ying
Mu, Yuguang
Yates, John R.
Drubin, David G.
author_sort Miao, Yansong
collection PubMed
description Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin–Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin's N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism.
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spelling pubmed-48320642016-04-25 Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast Miao, Yansong Han, Xuemei Zheng, Liangzhen Xie, Ying Mu, Yuguang Yates, John R. Drubin, David G. Nat Commun Article Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin–Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin's N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism. Nature Publishing Group 2016-04-12 /pmc/articles/PMC4832064/ /pubmed/27068241 http://dx.doi.org/10.1038/ncomms11265 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Miao, Yansong
Han, Xuemei
Zheng, Liangzhen
Xie, Ying
Mu, Yuguang
Yates, John R.
Drubin, David G.
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title_full Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title_fullStr Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title_full_unstemmed Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title_short Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
title_sort fimbrin phosphorylation by metaphase cdk1 regulates actin cable dynamics in budding yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832064/
https://www.ncbi.nlm.nih.gov/pubmed/27068241
http://dx.doi.org/10.1038/ncomms11265
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