(13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads

A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, (13)C single pulse excitation magic angle spinning NMR experiment with broadband (1)H decoupling is used to determine the peak integrals for a butyl ligand in the spectr...

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Autores principales: Elwinger, Fredrik, Dvinskikh, Sergey V., Furó, István
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832835/
https://www.ncbi.nlm.nih.gov/pubmed/26053054
http://dx.doi.org/10.1002/mrc.4257
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author Elwinger, Fredrik
Dvinskikh, Sergey V.
Furó, István
author_facet Elwinger, Fredrik
Dvinskikh, Sergey V.
Furó, István
author_sort Elwinger, Fredrik
collection PubMed
description A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, (13)C single pulse excitation magic angle spinning NMR experiment with broadband (1)H decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed. Copyright © 2015 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.
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spelling pubmed-48328352016-04-27 (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads Elwinger, Fredrik Dvinskikh, Sergey V. Furó, István Magn Reson Chem Research Articles A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, (13)C single pulse excitation magic angle spinning NMR experiment with broadband (1)H decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed. Copyright © 2015 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd. John Wiley and Sons Inc. 2015-06-06 2015-08 /pmc/articles/PMC4832835/ /pubmed/26053054 http://dx.doi.org/10.1002/mrc.4257 Text en Copyright © 2015 John Wiley & Sons, Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Elwinger, Fredrik
Dvinskikh, Sergey V.
Furó, István
(13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title_full (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title_fullStr (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title_full_unstemmed (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title_short (13)C SPE MAS measurement of ligand concentration in compressible chromatographic beads
title_sort (13)c spe mas measurement of ligand concentration in compressible chromatographic beads
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832835/
https://www.ncbi.nlm.nih.gov/pubmed/26053054
http://dx.doi.org/10.1002/mrc.4257
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