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Analytical workflow of double-digest restriction site-associated DNA sequencing based on empirical and in silico optimization in tomato

Double-digest restriction site-associated DNA sequencing (ddRAD-Seq) enables high-throughput genome-wide genotyping with next-generation sequencing technology. Consequently, this method has become popular in plant genetics and breeding. Although computational in silico prediction of restriction site...

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Detalles Bibliográficos
Autores principales: Shirasawa, Kenta, Hirakawa, Hideki, Isobe, Sachiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833422/
https://www.ncbi.nlm.nih.gov/pubmed/26932983
http://dx.doi.org/10.1093/dnares/dsw004