Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae

Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, pu...

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Detalles Bibliográficos
Autores principales: Kawakami, Hironori, Ohashi, Eiji, Tsurimoto, Toshiki, Katayama, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834435/
https://www.ncbi.nlm.nih.gov/pubmed/27148210
http://dx.doi.org/10.3389/fmicb.2016.00521

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834435/
https://www.ncbi.nlm.nih.gov/pubmed/27148210
http://dx.doi.org/10.3389/fmicb.2016.00521

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