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Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae
Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, pu...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834435/ https://www.ncbi.nlm.nih.gov/pubmed/27148210 http://dx.doi.org/10.3389/fmicb.2016.00521 |