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Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells

We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi...

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Autores principales: Rombo, Roman, Weiher, Hans, Schmidt-Wolf, Ingo G.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: German Medical Science GMS Publishing House 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836455/
https://www.ncbi.nlm.nih.gov/pubmed/27141211
http://dx.doi.org/10.3205/000231
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author Rombo, Roman
Weiher, Hans
Schmidt-Wolf, Ingo G.H.
author_facet Rombo, Roman
Weiher, Hans
Schmidt-Wolf, Ingo G.H.
author_sort Rombo, Roman
collection PubMed
description We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer.
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spelling pubmed-48364552016-05-02 Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells Rombo, Roman Weiher, Hans Schmidt-Wolf, Ingo G.H. Ger Med Sci Article We examined the cytotoxic effects of chaetocin on clear cell renal cell carcinoma (ccRCC) cells and the possibility to combine the effects of chaetocin with the effects of cytokine-induced killer cells (CIK) assayed by MTT assay and FACS analysis. Chaetocin is a thiodioxopiperazine produced by fungi belonging to the chaetomiaceae family. In 2007, it was first reported that chaetocin shows potent and selective ex vivo anti-cancer activity by inducing reactive oxygen species. CIK cells are generated from CD3+/CD56- T lymphocytes with double negative CD4-/CD8- phenotype that are isolated from human blood. The addition of distinct interleukins and antibodies results in the generation of CIK cells that are able to specifically target and destroy renal carcinoma cells. The results of this research state that the anti-ccRCC activity of chaetocin is weak and does not show a high grade of selectivity on clear cell renal cell carcinoma cells. Although the CIK cells show a high grade of selective anti-ccRCC activity, this effect could not be improved by the addition of chaetocin. So chaetocin seems to be no suitable agent for specific targeting ccRCC cells or for the combination therapy with CIK cells in renal cancer. German Medical Science GMS Publishing House 2016-04-18 /pmc/articles/PMC4836455/ /pubmed/27141211 http://dx.doi.org/10.3205/000231 Text en Copyright © 2016 Rombo et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License.
spellingShingle Article
Rombo, Roman
Weiher, Hans
Schmidt-Wolf, Ingo G.H.
Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title_full Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title_fullStr Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title_full_unstemmed Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title_short Effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
title_sort effect of chaetocin on renal cell carcinoma cells and cytokine-induced killer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836455/
https://www.ncbi.nlm.nih.gov/pubmed/27141211
http://dx.doi.org/10.3205/000231
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