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Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling
Positive selection occurs in the thymic cortex, but critical maturation events occur later in the medulla. We defined the precise stage at which T cells acquire competence to proliferate and emigrate. Transcriptome analysis of late gene changes suggested roles for NF-κB and interferon (IFN) signalin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837029/ https://www.ncbi.nlm.nih.gov/pubmed/27043411 http://dx.doi.org/10.1038/ni.3419 |
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author | Xing, Yan Wang, Xiaodan Jameson, Stephen C. Hogquist, Kristin A. |
author_facet | Xing, Yan Wang, Xiaodan Jameson, Stephen C. Hogquist, Kristin A. |
author_sort | Xing, Yan |
collection | PubMed |
description | Positive selection occurs in the thymic cortex, but critical maturation events occur later in the medulla. We defined the precise stage at which T cells acquire competence to proliferate and emigrate. Transcriptome analysis of late gene changes suggested roles for NF-κB and interferon (IFN) signaling. Mice lacking the IKK kinase TAK1 underwent normal positive selection, but exhibited a specific block in functional maturation. NF-κB signaling provided protection from tumor necrosis factor (TNF) mediated death, and was required for proliferation and emigration. The interferon signature was independent of NF-κB, however IFN-αR–deficient thymocytes showed reduced STAT1 expression and phenotypic abnormality, but were competent to proliferate. Thus, both NF-κB and tonic IFN signals are involved in the final maturation of thymocytes into naïve T cells. |
format | Online Article Text |
id | pubmed-4837029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
record_format | MEDLINE/PubMed |
spelling | pubmed-48370292016-10-04 Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling Xing, Yan Wang, Xiaodan Jameson, Stephen C. Hogquist, Kristin A. Nat Immunol Article Positive selection occurs in the thymic cortex, but critical maturation events occur later in the medulla. We defined the precise stage at which T cells acquire competence to proliferate and emigrate. Transcriptome analysis of late gene changes suggested roles for NF-κB and interferon (IFN) signaling. Mice lacking the IKK kinase TAK1 underwent normal positive selection, but exhibited a specific block in functional maturation. NF-κB signaling provided protection from tumor necrosis factor (TNF) mediated death, and was required for proliferation and emigration. The interferon signature was independent of NF-κB, however IFN-αR–deficient thymocytes showed reduced STAT1 expression and phenotypic abnormality, but were competent to proliferate. Thus, both NF-κB and tonic IFN signals are involved in the final maturation of thymocytes into naïve T cells. 2016-04-04 2016-05 /pmc/articles/PMC4837029/ /pubmed/27043411 http://dx.doi.org/10.1038/ni.3419 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Xing, Yan Wang, Xiaodan Jameson, Stephen C. Hogquist, Kristin A. Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title | Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title_full | Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title_fullStr | Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title_full_unstemmed | Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title_short | Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling |
title_sort | late stages of t cell maturation in the thymus involve nf-κb and tonic type i interferon signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837029/ https://www.ncbi.nlm.nih.gov/pubmed/27043411 http://dx.doi.org/10.1038/ni.3419 |
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