Quantification of transcription factor-DNA binding affinity in a living cell

The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...

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Detalles Bibliográficos
Autores principales: Belikov, Sergey, Berg, Otto G., Wrange, Örjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Gene regulation, Chromatin and Epigenetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838337/
https://www.ncbi.nlm.nih.gov/pubmed/26657626
http://dx.doi.org/10.1093/nar/gkv1350

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838337/
https://www.ncbi.nlm.nih.gov/pubmed/26657626
http://dx.doi.org/10.1093/nar/gkv1350

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