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Quantification of transcription factor-DNA binding affinity in a living cell
The apparent dissociation constant (K(d)) for specific binding of glucocorticoid receptor (GR) and androgen receptor (AR) to DNA was determined in vivo in Xenopus oocytes. The total nuclear receptor concentration was quantified as specifically retained [(3)H]-hormone in manually isolated oocyte nucl...
Autores principales: | Belikov, Sergey, Berg, Otto G., Wrange, Örjan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838337/ https://www.ncbi.nlm.nih.gov/pubmed/26657626 http://dx.doi.org/10.1093/nar/gkv1350 |
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