In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins

During evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. Comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequen...

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Detalles Bibliográficos
Autores principales: Shu, Changlong, Zhou, Jianqiao, Crickmore, Neil, Li, Xianchun, Song, Fuping, Liang, Gemei, He, Kanglai, Huang, Dafang, Zhang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838838/
https://www.ncbi.nlm.nih.gov/pubmed/27097519
http://dx.doi.org/10.1038/srep23536

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838838/
https://www.ncbi.nlm.nih.gov/pubmed/27097519
http://dx.doi.org/10.1038/srep23536

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