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In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins
During evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. Comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequen...
Autores principales: | Shu, Changlong, Zhou, Jianqiao, Crickmore, Neil, Li, Xianchun, Song, Fuping, Liang, Gemei, He, Kanglai, Huang, Dafang, Zhang, Jie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838838/ https://www.ncbi.nlm.nih.gov/pubmed/27097519 http://dx.doi.org/10.1038/srep23536 |
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