Construction and Periplasmic Expression of the Anti-EGFRvIII ScFv Antibody Gene in Escherichia coli

In the previous study, we constructed an expression vector carrying the anti-EGFRvIII scFv antibody gene with V(H)-linker-V(L) orientation. The proteins were successfully produced in the periplasmic space of Escherichia coli. In this study, we substituted the inserted DNA with V(L)-linker-V(H) orien...

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Detalles Bibliográficos
Autores principales: Dewi, Kartika Sari, Retnoningrum, Debbie Sofie, Riani, Catur, Fuad, Asrul Muhamad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Austrian Journal of Pharmaceutical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839555/
https://www.ncbi.nlm.nih.gov/pubmed/27110505
http://dx.doi.org/10.3797/scipharm.ISP.2015.06