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Construction and Periplasmic Expression of the Anti-EGFRvIII ScFv Antibody Gene in Escherichia coli
In the previous study, we constructed an expression vector carrying the anti-EGFRvIII scFv antibody gene with V(H)-linker-V(L) orientation. The proteins were successfully produced in the periplasmic space of Escherichia coli. In this study, we substituted the inserted DNA with V(L)-linker-V(H) orien...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Austrian Journal of Pharmaceutical Sciences
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839555/ https://www.ncbi.nlm.nih.gov/pubmed/27110505 http://dx.doi.org/10.3797/scipharm.ISP.2015.06 |