Using expected sequence features to improve basecalling accuracy of amplicon pyrosequencing data

BACKGROUND: Amplicon pyrosequencing targets a known genetic region and thus inherently produces reads highly anticipated to have certain features, such as conserved nucleotide sequence, and in the case of protein coding DNA, an open reading frame. Pyrosequencing errors, consisting mainly of nucleoti...

Descripción completa

Detalles Bibliográficos
Autores principales: Rask, Thomas S., Petersen, Bent, Chen, Donald S., Day, Karen P., Pedersen, Anders Gorm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841065/
https://www.ncbi.nlm.nih.gov/pubmed/27102804
http://dx.doi.org/10.1186/s12859-016-1032-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841065/
https://www.ncbi.nlm.nih.gov/pubmed/27102804
http://dx.doi.org/10.1186/s12859-016-1032-7

Ejemplares similares