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Using expected sequence features to improve basecalling accuracy of amplicon pyrosequencing data
BACKGROUND: Amplicon pyrosequencing targets a known genetic region and thus inherently produces reads highly anticipated to have certain features, such as conserved nucleotide sequence, and in the case of protein coding DNA, an open reading frame. Pyrosequencing errors, consisting mainly of nucleoti...
Autores principales: | Rask, Thomas S., Petersen, Bent, Chen, Donald S., Day, Karen P., Pedersen, Anders Gorm |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841065/ https://www.ncbi.nlm.nih.gov/pubmed/27102804 http://dx.doi.org/10.1186/s12859-016-1032-7 |
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