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Comparison of Boiling and Robotics Automation Method in DNA Extraction for Metagenomic Sequencing of Human Oral Microbes

The rapid improvement of next-generation sequencing performance now enables us to analyze huge sample sets with more than ten thousand specimens. However, DNA extraction can still be a limiting step in such metagenomic approaches. In this study, we analyzed human oral microbes to compare the perform...

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Detalles Bibliográficos
Autores principales: Yamagishi, Junya, Sato, Yukuto, Shinozaki, Natsuko, Ye, Bin, Tsuboi, Akito, Nagasaki, Masao, Yamashita, Riu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841512/
https://www.ncbi.nlm.nih.gov/pubmed/27104353
http://dx.doi.org/10.1371/journal.pone.0154389