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Comparison of Boiling and Robotics Automation Method in DNA Extraction for Metagenomic Sequencing of Human Oral Microbes
The rapid improvement of next-generation sequencing performance now enables us to analyze huge sample sets with more than ten thousand specimens. However, DNA extraction can still be a limiting step in such metagenomic approaches. In this study, we analyzed human oral microbes to compare the perform...
Autores principales: | Yamagishi, Junya, Sato, Yukuto, Shinozaki, Natsuko, Ye, Bin, Tsuboi, Akito, Nagasaki, Masao, Yamashita, Riu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841512/ https://www.ncbi.nlm.nih.gov/pubmed/27104353 http://dx.doi.org/10.1371/journal.pone.0154389 |
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