Homology-driven genome editing in hematopoietic stem and progenitor cells using zinc finger nuclease mRNA and AAV6 donors

Genome editing with targeted nucleases and DNA donor templates homologous to the break site has proven challenging in human hematopoietic stem and progenitor cells (HSPCs), and particularly in the most primitive, long-term repopulating cell population. Here we report that combining electroporation o...

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Detalles Bibliográficos
Autores principales: Wang, Jianbin, Exline, Colin M., DeClercq, Joshua J., Llewellyn, G. Nicholas, Hayward, Samuel B., Li, Patrick Wai-Lun, Shivak, David A., Surosky, Richard T., Gregory, Philip D., Holmes, Michael C., Cannon, Paula M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842001/
https://www.ncbi.nlm.nih.gov/pubmed/26551060
http://dx.doi.org/10.1038/nbt.3408

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842001/
https://www.ncbi.nlm.nih.gov/pubmed/26551060
http://dx.doi.org/10.1038/nbt.3408

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