Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...

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Detalles Bibliográficos
Autores principales: Wandrey, Georg, Bier, Claus, Binder, Dennis, Hoffmann, Kyra, Jaeger, Karl-Erich, Pietruszka, Jörg, Drepper, Thomas, Büchs, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://www.ncbi.nlm.nih.gov/pubmed/27107964
http://dx.doi.org/10.1186/s12934-016-0461-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842301/
https://www.ncbi.nlm.nih.gov/pubmed/27107964
http://dx.doi.org/10.1186/s12934-016-0461-3

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