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Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system
BACKGROUND: Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very labori...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842301/ https://www.ncbi.nlm.nih.gov/pubmed/27107964 http://dx.doi.org/10.1186/s12934-016-0461-3 |