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AB115. Effects of target gene expression on ex vivo differentiated mesenchymal stromal cells transfected by lentiviral vector with PDE5 of short hairpin RNA

OBJECTIVE: To investigate the expression of PDE5 in ex vivo differentiation of gene-modified BM-MSCs using the lentiviral vector containing silencing target gene PDE5 of shRNA. METHODS: SD rat bone marrow-derived MSCs were separated, cultured and purified in vitro by Percoll density gradient centrif...

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Detalles Bibliográficos
Autores principales: Xiao, Heng-Jun, Yan, Weixin, Chen, Jun, Gao, Xin, Zhuan, Li, Wang, Tao, Yang, Jun, Liu, Ji-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842601/
http://dx.doi.org/10.21037/tau.2016.s115