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A CRISPR/Cas9 system adapted for gene editing in marine algae

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen...

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Detalles Bibliográficos
Autores principales: Nymark, Marianne, Sharma, Amit Kumar, Sparstad, Torfinn, Bones, Atle M., Winge, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842962/
https://www.ncbi.nlm.nih.gov/pubmed/27108533
http://dx.doi.org/10.1038/srep24951