A CRISPR/Cas9 system adapted for gene editing in marine algae

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen...

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Autores principales: Nymark, Marianne, Sharma, Amit Kumar, Sparstad, Torfinn, Bones, Atle M., Winge, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842962/
https://www.ncbi.nlm.nih.gov/pubmed/27108533
http://dx.doi.org/10.1038/srep24951
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author Nymark, Marianne
Sharma, Amit Kumar
Sparstad, Torfinn
Bones, Atle M.
Winge, Per
author_facet Nymark, Marianne
Sharma, Amit Kumar
Sparstad, Torfinn
Bones, Atle M.
Winge, Per
author_sort Nymark, Marianne
collection PubMed
description Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.
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spelling pubmed-48429622016-04-29 A CRISPR/Cas9 system adapted for gene editing in marine algae Nymark, Marianne Sharma, Amit Kumar Sparstad, Torfinn Bones, Atle M. Winge, Per Sci Rep Article Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses. Nature Publishing Group 2016-04-25 /pmc/articles/PMC4842962/ /pubmed/27108533 http://dx.doi.org/10.1038/srep24951 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nymark, Marianne
Sharma, Amit Kumar
Sparstad, Torfinn
Bones, Atle M.
Winge, Per
A CRISPR/Cas9 system adapted for gene editing in marine algae
title A CRISPR/Cas9 system adapted for gene editing in marine algae
title_full A CRISPR/Cas9 system adapted for gene editing in marine algae
title_fullStr A CRISPR/Cas9 system adapted for gene editing in marine algae
title_full_unstemmed A CRISPR/Cas9 system adapted for gene editing in marine algae
title_short A CRISPR/Cas9 system adapted for gene editing in marine algae
title_sort crispr/cas9 system adapted for gene editing in marine algae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842962/
https://www.ncbi.nlm.nih.gov/pubmed/27108533
http://dx.doi.org/10.1038/srep24951
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