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A CRISPR/Cas9 system adapted for gene editing in marine algae
Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen...
Autores principales: | Nymark, Marianne, Sharma, Amit Kumar, Sparstad, Torfinn, Bones, Atle M., Winge, Per |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842962/ https://www.ncbi.nlm.nih.gov/pubmed/27108533 http://dx.doi.org/10.1038/srep24951 |
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