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Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis
We assessed exercise performance, coronary blood flow and cardiac reserve of female ApoE/LDLR(−/−) mice with advanced atherosclerosis compared with age-matched, wild-type C57BL6/J mice. Exercise capacity was assessed as whole body maximal oxygen consumption (V’O(2max)), maximum running velocity (v(m...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842974/ https://www.ncbi.nlm.nih.gov/pubmed/27108697 http://dx.doi.org/10.1038/srep24714 |
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author | Wojewoda, M. Tyrankiewicz, U. Gwozdz, P. Skorka, T. Jablonska, M. Orzylowska, A. Jasinski, K. Jasztal, A. Przyborowski, K. Kostogrys, R. B. Zoladz, J. A. Chlopicki, S. |
author_facet | Wojewoda, M. Tyrankiewicz, U. Gwozdz, P. Skorka, T. Jablonska, M. Orzylowska, A. Jasinski, K. Jasztal, A. Przyborowski, K. Kostogrys, R. B. Zoladz, J. A. Chlopicki, S. |
author_sort | Wojewoda, M. |
collection | PubMed |
description | We assessed exercise performance, coronary blood flow and cardiac reserve of female ApoE/LDLR(−/−) mice with advanced atherosclerosis compared with age-matched, wild-type C57BL6/J mice. Exercise capacity was assessed as whole body maximal oxygen consumption (V’O(2max)), maximum running velocity (v(max)) and maximum distance (DIST(max)) during treadmill exercise. Cardiac systolic and diastolic function in basal conditions and in response to dobutamine (mimicking exercise-induced cardiac stress) were assessed by Magnetic Resonance Imaging (MRI) in vivo. Function of coronary circulation was assessed in isolated perfused hearts. In female ApoE/LDLR(−/−) mice V’O(2max), v(max) and DIST(max) were not impaired as compared with C57BL6/J mice. Cardiac function at rest and systolic and diastolic cardiac reserve were also preserved in female ApoE/LDLR(−/−) mice as evidenced by preserved fractional area change and similar fall in systolic and end diastolic area after dobutamine. Moreover, endothelium-dependent responses of coronary circulation induced by bradykinin (Bk) and acetylcholine (ACh) were preserved, while endothelium-independent responses induced by NO-donors were augmented in female ApoE/LDLR(−/−) mice. Basal COX-2-dependent production of 6-keto-PGF(1α) was increased. Concluding, we suggest that robust compensatory mechanisms in coronary circulation involving PGI(2)- and NO-pathways may efficiently counterbalance coronary atherosclerosis-induced impairment in V’O(2max) and exercise capacity. |
format | Online Article Text |
id | pubmed-4842974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48429742016-04-29 Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis Wojewoda, M. Tyrankiewicz, U. Gwozdz, P. Skorka, T. Jablonska, M. Orzylowska, A. Jasinski, K. Jasztal, A. Przyborowski, K. Kostogrys, R. B. Zoladz, J. A. Chlopicki, S. Sci Rep Article We assessed exercise performance, coronary blood flow and cardiac reserve of female ApoE/LDLR(−/−) mice with advanced atherosclerosis compared with age-matched, wild-type C57BL6/J mice. Exercise capacity was assessed as whole body maximal oxygen consumption (V’O(2max)), maximum running velocity (v(max)) and maximum distance (DIST(max)) during treadmill exercise. Cardiac systolic and diastolic function in basal conditions and in response to dobutamine (mimicking exercise-induced cardiac stress) were assessed by Magnetic Resonance Imaging (MRI) in vivo. Function of coronary circulation was assessed in isolated perfused hearts. In female ApoE/LDLR(−/−) mice V’O(2max), v(max) and DIST(max) were not impaired as compared with C57BL6/J mice. Cardiac function at rest and systolic and diastolic cardiac reserve were also preserved in female ApoE/LDLR(−/−) mice as evidenced by preserved fractional area change and similar fall in systolic and end diastolic area after dobutamine. Moreover, endothelium-dependent responses of coronary circulation induced by bradykinin (Bk) and acetylcholine (ACh) were preserved, while endothelium-independent responses induced by NO-donors were augmented in female ApoE/LDLR(−/−) mice. Basal COX-2-dependent production of 6-keto-PGF(1α) was increased. Concluding, we suggest that robust compensatory mechanisms in coronary circulation involving PGI(2)- and NO-pathways may efficiently counterbalance coronary atherosclerosis-induced impairment in V’O(2max) and exercise capacity. Nature Publishing Group 2016-04-25 /pmc/articles/PMC4842974/ /pubmed/27108697 http://dx.doi.org/10.1038/srep24714 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wojewoda, M. Tyrankiewicz, U. Gwozdz, P. Skorka, T. Jablonska, M. Orzylowska, A. Jasinski, K. Jasztal, A. Przyborowski, K. Kostogrys, R. B. Zoladz, J. A. Chlopicki, S. Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title | Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title_full | Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title_fullStr | Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title_full_unstemmed | Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title_short | Exercise capacity and cardiac hemodynamic response in female ApoE/LDLR(−/−) mice: a paradox of preserved V’O(2max) and exercise capacity despite coronary atherosclerosis |
title_sort | exercise capacity and cardiac hemodynamic response in female apoe/ldlr(−/−) mice: a paradox of preserved v’o(2max) and exercise capacity despite coronary atherosclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842974/ https://www.ncbi.nlm.nih.gov/pubmed/27108697 http://dx.doi.org/10.1038/srep24714 |
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