Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors

Engineering the cofactor specificity of a natural enzyme often results in a significant decrease in its activity on original cofactor. Here we report that a NADH-dependent dehydrogenase (d-LDH) from Lactobacillus delbrueckii 11842 can be rationally engineered to efficiently use both NADH and NADPH a...

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Detalles Bibliográficos
Autores principales: Meng, Hengkai, Liu, Pi, Sun, Hongbing, Cai, Zhen, Zhou, Jie, Lin, Jianping, Li, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842997/
https://www.ncbi.nlm.nih.gov/pubmed/27109778
http://dx.doi.org/10.1038/srep24887

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842997/
https://www.ncbi.nlm.nih.gov/pubmed/27109778
http://dx.doi.org/10.1038/srep24887

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