Cargando…
Regulation of cell protrusions by small GTPases during fusion of the neural folds
Epithelial fusion is a crucial process in embryonic development, and its failure underlies several clinically important birth defects. For example, failure of neural fold fusion during neurulation leads to open neural tube defects including spina bifida. Using mouse embryos, we show that cell protru...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846376/ https://www.ncbi.nlm.nih.gov/pubmed/27114066 http://dx.doi.org/10.7554/eLife.13273 |
_version_ | 1782429053335109632 |
---|---|
author | Rolo, Ana Savery, Dawn Escuin, Sarah de Castro, Sandra C Armer, Hannah EJ Munro, Peter MG Molè, Matteo A Greene, Nicholas DE Copp, Andrew J |
author_facet | Rolo, Ana Savery, Dawn Escuin, Sarah de Castro, Sandra C Armer, Hannah EJ Munro, Peter MG Molè, Matteo A Greene, Nicholas DE Copp, Andrew J |
author_sort | Rolo, Ana |
collection | PubMed |
description | Epithelial fusion is a crucial process in embryonic development, and its failure underlies several clinically important birth defects. For example, failure of neural fold fusion during neurulation leads to open neural tube defects including spina bifida. Using mouse embryos, we show that cell protrusions emanating from the apposed neural fold tips, at the interface between the neuroepithelium and the surface ectoderm, are required for completion of neural tube closure. By genetically ablating the cytoskeletal regulators Rac1 or Cdc42 in the dorsal neuroepithelium, or in the surface ectoderm, we show that these protrusions originate from surface ectodermal cells and that Rac1 is necessary for the formation of membrane ruffles which typify late closure stages, whereas Cdc42 is required for the predominance of filopodia in early neurulation. This study provides evidence for the essential role and molecular regulation of membrane protrusions prior to fusion of a key organ primordium in mammalian development. DOI: http://dx.doi.org/10.7554/eLife.13273.001 |
format | Online Article Text |
id | pubmed-4846376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-48463762016-04-29 Regulation of cell protrusions by small GTPases during fusion of the neural folds Rolo, Ana Savery, Dawn Escuin, Sarah de Castro, Sandra C Armer, Hannah EJ Munro, Peter MG Molè, Matteo A Greene, Nicholas DE Copp, Andrew J eLife Cell Biology Epithelial fusion is a crucial process in embryonic development, and its failure underlies several clinically important birth defects. For example, failure of neural fold fusion during neurulation leads to open neural tube defects including spina bifida. Using mouse embryos, we show that cell protrusions emanating from the apposed neural fold tips, at the interface between the neuroepithelium and the surface ectoderm, are required for completion of neural tube closure. By genetically ablating the cytoskeletal regulators Rac1 or Cdc42 in the dorsal neuroepithelium, or in the surface ectoderm, we show that these protrusions originate from surface ectodermal cells and that Rac1 is necessary for the formation of membrane ruffles which typify late closure stages, whereas Cdc42 is required for the predominance of filopodia in early neurulation. This study provides evidence for the essential role and molecular regulation of membrane protrusions prior to fusion of a key organ primordium in mammalian development. DOI: http://dx.doi.org/10.7554/eLife.13273.001 eLife Sciences Publications, Ltd 2016-04-26 /pmc/articles/PMC4846376/ /pubmed/27114066 http://dx.doi.org/10.7554/eLife.13273 Text en © 2016, Rolo et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Rolo, Ana Savery, Dawn Escuin, Sarah de Castro, Sandra C Armer, Hannah EJ Munro, Peter MG Molè, Matteo A Greene, Nicholas DE Copp, Andrew J Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title | Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title_full | Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title_fullStr | Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title_full_unstemmed | Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title_short | Regulation of cell protrusions by small GTPases during fusion of the neural folds |
title_sort | regulation of cell protrusions by small gtpases during fusion of the neural folds |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846376/ https://www.ncbi.nlm.nih.gov/pubmed/27114066 http://dx.doi.org/10.7554/eLife.13273 |
work_keys_str_mv | AT roloana regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT saverydawn regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT escuinsarah regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT decastrosandrac regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT armerhannahej regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT munropetermg regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT molematteoa regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT greenenicholasde regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds AT coppandrewj regulationofcellprotrusionsbysmallgtpasesduringfusionoftheneuralfolds |