Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle

Blastocyst complementation (BC) systems have enabled in vivo generation of organs from allogeneic pluripotent cells, compensating for an empty germ cell niche in gene knockout (KO) animals. Here, we succeeded in producing chimeric beef cattle (Wagyu) by transferring allogenic germ cells into ovaries...

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Autores principales: Ideta, Atsushi, Yamashita, Shiro, Seki-Soma, Marie, Yamaguchi, Ryosaku, Chiba, Shiori, Komaki, Haruna, Ito, Tetsuya, Konishi, Masato, Aoyagi, Yoshito, Sendai, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846992/
https://www.ncbi.nlm.nih.gov/pubmed/27117862
http://dx.doi.org/10.1038/srep24983
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author Ideta, Atsushi
Yamashita, Shiro
Seki-Soma, Marie
Yamaguchi, Ryosaku
Chiba, Shiori
Komaki, Haruna
Ito, Tetsuya
Konishi, Masato
Aoyagi, Yoshito
Sendai, Yutaka
author_facet Ideta, Atsushi
Yamashita, Shiro
Seki-Soma, Marie
Yamaguchi, Ryosaku
Chiba, Shiori
Komaki, Haruna
Ito, Tetsuya
Konishi, Masato
Aoyagi, Yoshito
Sendai, Yutaka
author_sort Ideta, Atsushi
collection PubMed
description Blastocyst complementation (BC) systems have enabled in vivo generation of organs from allogeneic pluripotent cells, compensating for an empty germ cell niche in gene knockout (KO) animals. Here, we succeeded in producing chimeric beef cattle (Wagyu) by transferring allogenic germ cells into ovaries using somatic cell nuclear transfer and BC technology. The KO of NANOS3 (NANOS3(−/−)) in Wagyu bovine ovaries produced a complete loss of germ cells. Holstein blastomeres (NANOS3(+/+)) were injected into NANOS3(−/−) Wagyu embryos. Subsequently, exogenous germ cells (NANOS3(+/+)) were identified in the NANOS3(−/−) ovary. These results clearly indicate that allogeneic germ cells can be generated in recipient germ cell-free gonads using cloning and BC technologies.
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spelling pubmed-48469922016-05-04 Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle Ideta, Atsushi Yamashita, Shiro Seki-Soma, Marie Yamaguchi, Ryosaku Chiba, Shiori Komaki, Haruna Ito, Tetsuya Konishi, Masato Aoyagi, Yoshito Sendai, Yutaka Sci Rep Article Blastocyst complementation (BC) systems have enabled in vivo generation of organs from allogeneic pluripotent cells, compensating for an empty germ cell niche in gene knockout (KO) animals. Here, we succeeded in producing chimeric beef cattle (Wagyu) by transferring allogenic germ cells into ovaries using somatic cell nuclear transfer and BC technology. The KO of NANOS3 (NANOS3(−/−)) in Wagyu bovine ovaries produced a complete loss of germ cells. Holstein blastomeres (NANOS3(+/+)) were injected into NANOS3(−/−) Wagyu embryos. Subsequently, exogenous germ cells (NANOS3(+/+)) were identified in the NANOS3(−/−) ovary. These results clearly indicate that allogeneic germ cells can be generated in recipient germ cell-free gonads using cloning and BC technologies. Nature Publishing Group 2016-04-27 /pmc/articles/PMC4846992/ /pubmed/27117862 http://dx.doi.org/10.1038/srep24983 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ideta, Atsushi
Yamashita, Shiro
Seki-Soma, Marie
Yamaguchi, Ryosaku
Chiba, Shiori
Komaki, Haruna
Ito, Tetsuya
Konishi, Masato
Aoyagi, Yoshito
Sendai, Yutaka
Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title_full Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title_fullStr Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title_full_unstemmed Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title_short Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle
title_sort generation of exogenous germ cells in the ovaries of sterile nanos3-null beef cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846992/
https://www.ncbi.nlm.nih.gov/pubmed/27117862
http://dx.doi.org/10.1038/srep24983
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