Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing

The CRISPR/Cas9 system has been developed as an easy-handle and multiplexable approach for engineering eukaryotic genomes by zygote microinjection of Cas9 and sgRNA, while preparing Cas9 for microinjection is laborious and introducing inconsistency into the experiment. Here, we describe a modified s...

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Detalles Bibliográficos
Autores principales: Zhang, Linlin, Zhou, Jiankui, Han, Jinxiong, Hu, Bian, Hou, Ningning, Shi, Yun, Huang, Xingxu, Lou, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847922/
https://www.ncbi.nlm.nih.gov/pubmed/27119535
http://dx.doi.org/10.1371/journal.pone.0154364

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847922/
https://www.ncbi.nlm.nih.gov/pubmed/27119535
http://dx.doi.org/10.1371/journal.pone.0154364

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