Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices

We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 3...

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Detalles Bibliográficos
Autores principales: Yuan, Feng, Xiong, Guoxiang, Cohen, Noam A., Cohen, Akiva S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2017
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848166/
https://www.ncbi.nlm.nih.gov/pubmed/26509907
http://dx.doi.org/10.1097/PAI.0000000000000293

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848166/
https://www.ncbi.nlm.nih.gov/pubmed/26509907
http://dx.doi.org/10.1097/PAI.0000000000000293

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