Cargando…
Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices
We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 3...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Lippincott Williams & Wilkins
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848166/ https://www.ncbi.nlm.nih.gov/pubmed/26509907 http://dx.doi.org/10.1097/PAI.0000000000000293 |