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Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan
Programmed cell death-1 (PD-1) is a member of the CD28 superfamily that delivers negative signals on interaction with its 2 ligands, PD-L1 and PD-L2. We assessed the contribution of the PD-1 pathway to regulating the polarization of macrophages that promote inflammation induced by zymosan. We found...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849159/ https://www.ncbi.nlm.nih.gov/pubmed/26913605 http://dx.doi.org/10.1038/cddis.2016.33 |
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author | Chen, W Wang, J Jia, L Liu, J Tian, Y |
author_facet | Chen, W Wang, J Jia, L Liu, J Tian, Y |
author_sort | Chen, W |
collection | PubMed |
description | Programmed cell death-1 (PD-1) is a member of the CD28 superfamily that delivers negative signals on interaction with its 2 ligands, PD-L1 and PD-L2. We assessed the contribution of the PD-1 pathway to regulating the polarization of macrophages that promote inflammation induced by zymosan. We found that PD-1(−/−) mice developed robust peritonitis with more abundant infiltration of M1 macrophages, accompanied by higher levels of pro-inflammation factors, especially monocyte chemotactic protein-1 (MCP-1) compared with wild-type controls ex vivo and in vitro. Our results indicated that PD-1 deficiency promotes M1 rather than M2 polarization of macrophages by enhancing the expression of p-STAT1/p-NF-κB p65 and downregulating p-STAT6. We found that PD-1 engagement followed by zymosan stimulation might primarily attenuate the phosphorylation of tyrosine residue in PD-1 receptor/ligand and the recruitment of SHP-2 to PD-1 receptor/ligand, leading to the reduction of M1 type cytokine production. |
format | Online Article Text |
id | pubmed-4849159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48491592016-05-10 Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan Chen, W Wang, J Jia, L Liu, J Tian, Y Cell Death Dis Original Article Programmed cell death-1 (PD-1) is a member of the CD28 superfamily that delivers negative signals on interaction with its 2 ligands, PD-L1 and PD-L2. We assessed the contribution of the PD-1 pathway to regulating the polarization of macrophages that promote inflammation induced by zymosan. We found that PD-1(−/−) mice developed robust peritonitis with more abundant infiltration of M1 macrophages, accompanied by higher levels of pro-inflammation factors, especially monocyte chemotactic protein-1 (MCP-1) compared with wild-type controls ex vivo and in vitro. Our results indicated that PD-1 deficiency promotes M1 rather than M2 polarization of macrophages by enhancing the expression of p-STAT1/p-NF-κB p65 and downregulating p-STAT6. We found that PD-1 engagement followed by zymosan stimulation might primarily attenuate the phosphorylation of tyrosine residue in PD-1 receptor/ligand and the recruitment of SHP-2 to PD-1 receptor/ligand, leading to the reduction of M1 type cytokine production. Nature Publishing Group 2016-02 2016-02-25 /pmc/articles/PMC4849159/ /pubmed/26913605 http://dx.doi.org/10.1038/cddis.2016.33 Text en Copyright © 2016 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Article Chen, W Wang, J Jia, L Liu, J Tian, Y Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title | Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title_full | Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title_fullStr | Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title_full_unstemmed | Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title_short | Attenuation of the programmed cell death-1 pathway increases the M1 polarization of macrophages induced by zymosan |
title_sort | attenuation of the programmed cell death-1 pathway increases the m1 polarization of macrophages induced by zymosan |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849159/ https://www.ncbi.nlm.nih.gov/pubmed/26913605 http://dx.doi.org/10.1038/cddis.2016.33 |
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