Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9

CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mutations. To fulfill this promise, a strategy that effects single nucleotide changes whil...

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Detalles Bibliográficos
Autores principales: Biot-Pelletier, Damien, Martin, Vincent J. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850645/
https://www.ncbi.nlm.nih.gov/pubmed/27134651
http://dx.doi.org/10.1186/s13036-016-0028-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850645/
https://www.ncbi.nlm.nih.gov/pubmed/27134651
http://dx.doi.org/10.1186/s13036-016-0028-1

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