AID up-mutants isolated using a high-throughput screen highlight the immunity/cancer balance limiting DNA deaminase activity

DNA deaminases underpin pathways in antibody diversification (AID) and anti-viral immunity (APOBEC3s). Here we show how a high-throughput bacterial papillation assay can be used to screen for AID mutants with increased catalytic activity. The upmutations focus on a small number of residues, some hig...

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Detalles Bibliográficos
Autores principales: Wang, Meng, Yang, Zizhen, Rada, Cristina, Neuberger, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851237/
https://www.ncbi.nlm.nih.gov/pubmed/19543289
http://dx.doi.org/10.1038/nsmb.1623

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851237/
https://www.ncbi.nlm.nih.gov/pubmed/19543289
http://dx.doi.org/10.1038/nsmb.1623

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