Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption

Cell-free protein synthesis (CFPS) is a powerful tool for the preparation of toxic proteins, directed protein evolution, and bottom-up synthetic biology. The transcription-translation machinery for CFPS is provided by cell extracts, which usually contain 20–30 mg/mL of proteins. In general, these ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujiwara, Kei, Doi, Nobuhide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851396/
https://www.ncbi.nlm.nih.gov/pubmed/27128597
http://dx.doi.org/10.1371/journal.pone.0154614

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851396/
https://www.ncbi.nlm.nih.gov/pubmed/27128597
http://dx.doi.org/10.1371/journal.pone.0154614

Ejemplares similares