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Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption
Cell-free protein synthesis (CFPS) is a powerful tool for the preparation of toxic proteins, directed protein evolution, and bottom-up synthetic biology. The transcription-translation machinery for CFPS is provided by cell extracts, which usually contain 20–30 mg/mL of proteins. In general, these ce...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851396/ https://www.ncbi.nlm.nih.gov/pubmed/27128597 http://dx.doi.org/10.1371/journal.pone.0154614 |