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Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX
OBJECTIVES: To identify the best lipid nanoparticles for delivery of purified Cas9 protein and gRNA complexes (Cas9 RNPs) into mammalian cells and to establish the optimal conditions for transfection. RESULTS: Using a systematic approach, we screened 60 transfection reagents using six commonly-used...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853464/ https://www.ncbi.nlm.nih.gov/pubmed/26892225 http://dx.doi.org/10.1007/s10529-016-2064-9 |