Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX

OBJECTIVES: To identify the best lipid nanoparticles for delivery of purified Cas9 protein and gRNA complexes (Cas9 RNPs) into mammalian cells and to establish the optimal conditions for transfection. RESULTS: Using a systematic approach, we screened 60 transfection reagents using six commonly-used...

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Detalles Bibliográficos
Autores principales: Yu, Xin, Liang, Xiquan, Xie, Huimin, Kumar, Shantanu, Ravinder, Namritha, Potter, Jason, de Mollerat du Jeu, Xavier, Chesnut, Jonathan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853464/
https://www.ncbi.nlm.nih.gov/pubmed/26892225
http://dx.doi.org/10.1007/s10529-016-2064-9

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