New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates

To further expand the substrate range of the cyclohexylamine oxidase (CHAO) from Brevibacterium oxydans, a library of diverse mutants was created and assayed toward a group of structurally diverse substrates. Among them, mutants T198A and M226A exhibited enhanced activity relative to wt CHAO for mos...

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Detalles Bibliográficos
Autores principales: Li, Guangyue, Yao, Peiyuan, Cong, Peiqian, Ren, Jie, Wang, Lei, Feng, Jinhui, Lau, Peter C.K., Wu, Qiaqing, Zhu, Dunming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853734/
https://www.ncbi.nlm.nih.gov/pubmed/27138090
http://dx.doi.org/10.1038/srep24973

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853734/
https://www.ncbi.nlm.nih.gov/pubmed/27138090
http://dx.doi.org/10.1038/srep24973

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