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Development of Quantitative Real-time PCR Assays for Different Clades of “Candidatus Accumulibacter”

We designed novel quantitative real-time polymerase chain reaction (qPCR) primers for the polyphosphate kinase 1 (ppk1) gene, targeting eight individual “Candidatus Accumulibacter” (referred to as Accumulibacter) clades. An evaluation of primer sets was conducted regarding the coverage, specificity,...

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Detalles Bibliográficos
Autores principales: Zhang, An Ni, Mao, Yanping, Zhang, Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855135/
https://www.ncbi.nlm.nih.gov/pubmed/27142574
http://dx.doi.org/10.1038/srep23993