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Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci

In order to elucidate the functional organization of the genome, it is vital to directly visualize the interactions between genomic elements in living cells. For this purpose, we engineered the Cas9 protein from Staphylococcus aureus (SaCas9) for the imaging of endogenous genomic loci, which showed...

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Detalles Bibliográficos
Autores principales: Chen, Baohui, Hu, Jeffrey, Almeida, Ricardo, Liu, Harrison, Balakrishnan, Sanjeev, Covill-Cooke, Christian, Lim, Wendell A., Huang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856973/
https://www.ncbi.nlm.nih.gov/pubmed/26740581
http://dx.doi.org/10.1093/nar/gkv1533