In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining

The CRISPR/Cas9 system facilitates precise DNA modifications by generating RNA-guided blunt-ended double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called...

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Detalles Bibliográficos
Autores principales: Geisinger, Jonathan M., Turan, Sören, Hernandez, Sophia, Spector, Laura P., Calos, Michele P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856974/
https://www.ncbi.nlm.nih.gov/pubmed/26762978
http://dx.doi.org/10.1093/nar/gkv1542

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856974/
https://www.ncbi.nlm.nih.gov/pubmed/26762978
http://dx.doi.org/10.1093/nar/gkv1542

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