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In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining
The CRISPR/Cas9 system facilitates precise DNA modifications by generating RNA-guided blunt-ended double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856974/ https://www.ncbi.nlm.nih.gov/pubmed/26762978 http://dx.doi.org/10.1093/nar/gkv1542 |